CRISPR-Cas: A Laboratory Manual by Jennifer Doudna

• CRISPR-Cas: A Laboratory Manual
• Jennifer Doudna
• Page: 450
• Format: pdf, ePub, mobi, fb2
• ISBN: 9781621821311
• Publisher: Cold Spring Harbor Laboratory Press

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CRISPR-Cas-based techniques are revolutionizing the way geneticists and molecular biologists modify DNA sequences and modulate gene expression in cells and organisms. This laboratory manual presents step-by-step protocols for applying this cutting-edge technology to any system of interest. Contributors describe approaches for designing guide RNAs that precisely target the gene of interest, delivering CRISPR-Cas reagents into cells (e.g., using AAV vectors), and selecting cells that harbor the desired changes. Strategies for scaling and multiplexing these procedures are also described, as are methods for optimizing CRISPR-Cas systems in common species and cell lines, from yeast to human.

CRISPR-Cas: A Laboratory Manual: 9781621821304: Medicine
Featuring contributions from leading figures in the CRISPR-Cas field, this laboratory manual presents a state-of-the-art guide to the technology. It includes   The CRISPR web page - CSIC
Lluis Montoliu's lab at CNB-CSIC, Madrid, Spain. Multiplex genome engineering using CRISPR/Cas systems. Cong L, Ran FA, Cox D, Lin S,  Adenoviral vector delivery of RNA-guided CRISPR/Cas9 nuclease
CRISPR/Cas9-derived RNA-guided nucleases (RGNs) are DNA A Laboratory Manual, 3rd Ed. Sambrook, J. F. & Russell, D. W. (eds.)  CRISPR-Cas: A Laboratory Manual: 9781621821311: Medicine
Featuring contributions from leading figures in the CRISPR-Cas field, this laboratory manual presents a state-of-the-art guide to the technology. It includes   The Streptococcus thermophilus CRISPR/Cas system provides
The CRISPR/Cas adaptive immune system provides resistance against phages and plasmids in Molecular Cloning: A Laboratory Manual. Cold Spring Harbor Lab Press Genomics
CRISPR-Cas: A Laboratory Manual Edited By Jennifer Doudna, HHMI/University of California Berkeley; Prashant Mali, University of California San Diego. Cold Spring Harbor Lab Press Biochemistry
CRISPR-Cas: A Laboratory Manual Edited By Jennifer Doudna, HHMI/University of California Berkeley; Prashant Mali, University of California San Diego. Cold Spring Harbor Lab Press Molecular Biology
CRISPR-Cas: A Laboratory Manual Edited By Jennifer Doudna, HHMI/University of California Berkeley; Prashant Mali, University of California San Diego. Cold Spring Harbor Lab Press Molecular Biology
CRISPR-Cas: A Laboratory Manual Edited By Jennifer Doudna, HHMI/University of California Berkeley; Prashant Mali, University of California San Diego. Cold Spring Harbor Lab Press Books
CRISPR-Cas: A Laboratory Manual Edited By Jennifer Doudna, HHMI/University of California Berkeley; Prashant Mali, University of California San Diego. 2 - Cold Spring Harbor Lab Press
CRISPR-Cas: A Laboratory Manual Edited By Jennifer Doudna, HHMI/University of California Berkeley; Prashant Mali, University of California San Diego. Cold Spring Harbor Lab Press Laboratory Techniques
CRISPR-Cas: A Laboratory Manual Edited By Jennifer Doudna, HHMI/University of California Berkeley; Prashant Mali, University of California San Diego. Cold Spring Harbor Lab Press Audiovisual
CRISPR-Cas: A Laboratory Manual Edited By Jennifer Doudna, HHMI/University of California Berkeley; Prashant Mali, University of California San Diego.

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